Figure 3. Structures of McRI_mecD-1 and McRI_mecD-2 and flanking sequences.

Comparison was performed with sequences of M. caseolyticus strains JCSC5402 (Genbank acc. no. NC_011999, position 215180–250630), IMD0473 (Genbank acc. no KY013610), IMD0819 (Genbank acc. no KY013611), KM0211 (Genbank acc. no KY013612) and KM1352 (Genbank acc. no KY013613) using Easyfig software⁵⁷. Gray areas indicate regions with between 68% to 100% nucleotide sequence identity. Regions encompassing McRI_mecD-1, McRI_mecD-2 and McCI_IMD0819 are indicated by horizontal black lines and flanking direct repeats (DRs) by vertical blue lines. The open reading frames (orfs) are represented by arrows: mecD, mecR1_m and mecI_m are shown in red, orfs encoding integrase (int) or transposase (tnp) are shown in yellow, reverse transciptases (rt) in beige, orfs associated with restriction-modification in green and virulence-associated orfs in mauve; the orfs occurring in all M. caseolyticus strains are shown in black and additional strain-specific orfs in blue. The primers used in this study are indicated by small black arrowheads (a), mecD-F; (b), mecD-R; (c), truA-F; (d), s66-R; (e), int-0819-F; (f), int-0473-F; (g), araC-F; (h), orf21-R; (i), orf20-F; (j), cop-R) and cleavage sites for restriction endonucleases HindIII (H3) and HincII (H2) and EcoRI (E1) by thin vertical lines.