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. 2017 Mar 8;7:43893. doi: 10.1038/srep43893

Figure 1. siRNA screening identifies six human genes required for the replication of influenza virus.

Figure 1

(A) Schematic representation of the screening procedure. (B) Scatter plot distribution of the duplicate screening assays. Virus replication was quantified by measuring percentage of GFP-positive cells and normalized to that of siScr-transfected cells and that of siCSE1L transfected cells, which were set 100% and 0%, respectively. The red box indicates hits meeting the selection criteria and the Z’ factors indicated on each diagram are averaged from the duplicated screens of ten 384-well plates. (C) Venn diagram of hits validated in duplicate screenings, indicating six overlapping hits representing genes of interest. (D) The six siRNAs targeting the indicated genes inhibit the replication of rPR8-GFP virus by more than 50%. Error bars are representative of standard deviation (SD) from duplicate screenings. All reductions are significant (p < 0.05, Student’s t-test).