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. 2017 Mar 8;7:44126. doi: 10.1038/srep44126

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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An unrooted neighbor joining tree of M. koenigii and other terpene synthases with bootstrap value of 1000 runs. The color shaded boxes represent TPS subfamilies a–g. The characterized TPSs in this study are boxed in blue. AbLIMS, Abies grandis (−)-4S-limonene synthase; AaLIS, Actinidia arguta linalool synthase; AdGDS, Actinidia deliciosa germacrene-D synthase; AmNES/LIS-1, Antirrhinum majus nerolidol/linalool synthase 1; AtTPS02, Arabidopsis thaliana terpene synthase 02; AtTPS10, A. thaliana terpene synthase10; CsαPINS, Cannabis sativa (+)-α-pinene synthase; CjGES, Citrus jambhiri geraniol synthase; RlemTPS2, C. jambhiri sabinene synthase; RlemTPS4, C. jambhiri δ-elemene synthase; CjβPINS, C. jambhiri β- pinene synthase; CjuβFS, C. junos (E)-β-farnesene synthase; ClLIMS2, C. limon limonene synthase 2; ClβPINS, C. limon (−)-β-pinene synthase; ClγTPNS, C. limon γ-terpinene synthase; CsiVLS, C. sinensis valencene synthase; CudLIMS, C. unshiu limonene synthase; CuLIS, C. unshiu linalool synthase; CuβOCS, C. unshiu (E)-β-ocimene synthase; CuγTPNS, C. unshiu γ-terpinene synthase; CbLIS2, Clarkia breweri linalool synthase 2; CcLIS, C. concinna linalool synthase; Cmα-FS1, Cucumis melo α-farnesene synthase; CsαFS, C. sativus (E,E)-α-farnesene synthase; CmCPS, Cucurbita maxima copalyl diphosphate sythase; GaδCDS, Gossypium arboreum (+)-δ-cadinene synthase; HcMTPS7, Hedychium coronarium chloroplast monoterpene synthase; MdαFS, Malus domestica (E,E)-α-farnesene synthase; MpβFS, Mentha piperita β-farnesene synthase; ObGES, Ocimum basilicum geraniol synthase; OsKSL10, Oryza sativa Ent-sandaracopimaradiene synthase; OfTPS4, Osmanthus fragrans α-farnesene synthase; Ppent-KS, Physcomitrella patens (−)-ent-kaurene synthase; PaαBS, Picea abies E-α-bisabolene synthase; PaLIS, P. abies (−)-linalool synthase; PaαFS, P. abies (E,E)-α-farnesene synthase; PsLIMS, P. sitchensis (−)-limonene synthase; PsPINS, P. sitchensis pinene synthase; PsSS, P. sitchensis (+)-sabinene synthase; Pinus sylvestris E-β-farnesene synthase; PtαFS Psβ-FS, Pinus taeda α-farnesene synthase; Ptα-FS, P. taeda α-farnesene synthase; PaIPS, Populus alba isoprene synthase; PtIPS, P. tremuloides isoprene synthase; Rcα-FS, Ricinus communis α-farnesene synthase; SoSS, Salvia officinalis (+)-sabinene synthase; SrCPS, Stevia rebaudiana copalyl pyrophosphate synthase; TpSS, Thuja plicata sabinene synthase; Vvα-FS1, Vitis vinifera α-farnesene synthase; VvαFS2, V. vinifera α-farnesene/β-ocimene synthase; VvVLS, V. vinifera valencene synthase. Accession numbers of protein sequences used in this figure are given in Supplementary Information.