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. 2017 Mar 8;7:44126. doi: 10.1038/srep44126

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Copyright © 2017, The Author(s)

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(a) GC-MS chromatogram with mass spectra for the product formed by MkTPS1 enzyme from geranyl diphosphate (GPP). Terpene synthase assay was carried out using the Ni-NTA purified MkTPS1 in the presence of 70 μM GPP as substrate. Boiled protein was used as control. Reaction products were obtained by solid-phase microextraction (SPME) and analyzed by GC-MS. (b) Choromatograms of chiral GC analysis using authentic standards, leaf essential oil, and MkTPS1 assay product. The numbers labeled in the chromatogram are as follows: 1, (−)-α-pinene; 2, (+)-α-pinene; 3, (+)-β-pinene; 4, (−)-β-pinene; 5, (+)-sabinene; 6, (−)-sabinene.