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. 2017 Mar 8;7:44126. doi: 10.1038/srep44126

Figure 7. Analysis of reaction products formed by recombinant MkTPS2 from farnesyl diphosphate (FPP).

Figure 7

(a) GC-MS chromatogram showing the product formed by MkTPS2 enzyme from FPP. (b) Mass spectra of α-farnesene. Terpene synthase assay was carried out using the purified recombinant MkTPS2 in the presence of 70 μM FPP as substrate. Boiled protein was used as control. Reaction products were obtained by solid-phase microextraction (SPME) and analyzed by GC-MS. (c) GC chromatograms of authentic farnesene standard mix (black) and MkTPS2 enzymatic product (green).