. 2017 Mar 8;7:43833. doi: 10.1038/srep43833

Table 2. Comparison of recovery and viability of isolated cells between the two protocols.

Sample group	Blood volume (ml)	Total WBC count in CBC (10³/μl)^*	Isolated WBCs by the hemolytic protocol		Isolated PBMCs by the gradient protocol		Viability comparison P-value^***
Sample group	Blood volume (ml)	Total WBC count in CBC (10³/μl)^*	Recovery^**	Viability^**	Recovery^**	Viability^**	Viability comparison P-value^***
#1	5	6.2 ± 1.2	47.4 ± 3.8	97.7 ± 1.3	18.4 ± 1.8	98.4 ± 1.7	0.1140
#2	2	5.6 ± 1.4	46.1 ± 4.1	97.1 ± 1.2	16.2 ± 2.1	98.1 ± 3.5	0.1925
#3	0.1	5.7 ± 1.3	41.6 ± 4.4	96.6 ± 1.5	14.6 ± 2.4	98.8 ± 5.6	0.0012

^*Values indicate the mean ± SD of total cell number of WBC in the CBC.

^*Values represented as the mean ± SD of the percentage.

^***P-values indicate the comparison between the hemolytic and the gradient protocol by Wilcoxon signed-rank test.