Table 3. Proportion of cell components of the isolated cells.

Protocol	Sample group	Blood volume (ml)	WBCs
			Granulocytes*			PBMCs*
			Neutrophils	Eosinophils	Basophils	Lymphocytes	Monocytes
Hemolytic protocol	#1	5	52.8 ± 5.7	2.1 ± 1.3	0.4 ± 0.5	43.6 ± 5.3	1.2 ± 0.7
	#2	2	53.8 ± 6.9	1.5 ± 1.2	0.6 ± 0.5	43.1 ± 6.5	1.1 ± 0.8
	#3	0.1	52.3 ± 4.5	3.4 ± 1.7	0.6 ± 0.5	42.8 ± 4.7	1.1 ± 0.8
Gradient protocol	#1	5	8.2 ± 3.5	0.8 ± 0.7	0.2 ± 0.4	90.2 ± 4.3	0.9 ± 0.6
	#2	2	15.6 ± 5.0	0.8 ± 0.6	0.2 ± 0.4	82.6 ± 5.5	0.9 ± 0.7
	#3	0.1	30.2 ± 6.9	1.6 ± 0.8	0.3 ± 0.5	67.2 ± 7.1	0.8 ± 0.6

^*Values represent the mean ± SD of the percentage of the cells in each sample group.