Table 4. Quality and quantity of genomic DNA for genotype concordance analyses.
| Sample group | Blood volume (ml) | DNA source | −80 °C period (weeks) | Cell number when thawed (×106) | Viability when thawed (%) | Culture period (weeks)**** | Final cell number (×106) | DNA yield (μg) | OD260/280 |
|---|---|---|---|---|---|---|---|---|---|
| #7 | 5 | Peripheral blood* | 136** | — | — | — | — | 4.2 ± 1.9 | 1.79 ± 0.09 |
| LCL-gradient | 130*** | 1.2 ± 4.9 | 79.2 ± 4.7 | 6 | 2.3 ± 0.4 | 3.2 ± 0.7 | 1.96 ± 0.02 | ||
| LCL-hemolytic | 130*** | 1.1 ± 2.2 | 80.2 ± 5.5 | 6 | 3.0 ± 0.4 | 3.8 ± 1.3 | 1.89 ± 0.01 | ||
| #8 | 2 | Peripheral blood* | 60** | — | — | — | — | 3.2 ± 2.2 | 1.82 ± 0.03 |
| LCL-hemolytic | 52*** | 0.4 ± 0.4 | 78.9 ± 8.3 | 8 | 2.0 ± 1.0 | 2.6 ± 1.2 | 1.93 ± 0.04 | ||
| #9 | 0.1 | Peripheral blood* | 10** | — | — | — | — | 3.7 ± 3.5 | 1.82 ± 0.04 |
| LCL-hemolytic | 0 | — | — | 12 | 0.7 ± 0.2 | 1.7 ± 0.6 | 1.88 ± 0.01 |
*350 ul of peripheral blood was used as a source of DNA.
**Genomic DNA was stored at −80 °C.
***LCLs were stored at −80 °C after 2 weeks culture from EBV infection.
****Total cultivation time including 2 weeks culture before cryopreservation.
Except for the “−80 °C period” and the “Cultivation period”, the values represent the mean ± SD.