Table 5. Cross-classification of genotype calls for evaluating the protocols started from 5 ml of peripheral blood.
Compared sample | Peripheral blood from sample group #7 (All SNPs) |
Concordance (%) | Kappa** | |||||
---|---|---|---|---|---|---|---|---|
AA | AB | BB | No call | Total | ||||
Peripheral blood from sample group #7 (as technical replicate) | AA | 127,257 | 57 | 0 | 626 | 127,940 | ||
AB | 129 | 89,987 | 88 | 545 | 90,749 | |||
BB | 0 | 61 | 126,041 | 622 | 126,724 | |||
No Call | 936 | 509 | 859 | 895 | 3,199 | |||
Total | 128,322 | 90,614 | 126,988 | 2,688 | 348,612* | 99.90 | 0.9985 | |
LCL-hemolytic from sample group #7 | AA | 127,613 | 68 | 0 | 647 | 128,328 | ||
AB | 80 | 90,031 | 62 | 506 | 90,679 | |||
BB | 0 | 78 | 126,305 | 644 | 127,027 | |||
No Call | 629 | 437 | 621 | 891 | 2,578 | |||
Total | 128,322 | 90,614 | 126,988 | 2,688 | 348,612* | 99.92 | 0.9987 | |
LCL-gradient from sample group #7 | AA | 127,139 | 52 | 0 | 568 | 127,759 | ||
AB | 125 | 89,964 | 140 | 602 | 90,831 | |||
BB | 0 | 77 | 125,774 | 548 | 126,399 | |||
No Call | 1,058 | 521 | 1,074 | 970 | 3,623 | |||
Total | 128,322 | 90,614 | 126,988 | 2,688 | 348,612* | 99.89 | 0.9983 |
*Affy100k Array (116,204 SNPs) × 3 samples = 348,612 SNPs.
**Closer to 1.0 indicates higher reproducibility.