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. 2017 Mar 8;7:44064. doi: 10.1038/srep44064

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Copyright © 2017, The Author(s)

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(A) PMEL fibril enrichment via velocity gradient centrifugation coupled with subsequent Triton X-100 extraction of all fractions. Triton X-100-insoluble material from each fraction was analyzed by Western blot. (B) Band intensities in (A) were determined densitometrically (lines). MαC:RPT ratios are shown as filled histogram. (C) SDS-PAGE and Coomassie Blue staining of proto_ΙΙ and mature fractions. (D) A mature fraction analyzed by Western blot and Coomassie Blue staining.