Figure 5. Various PMEL loss-of-function mutants are defective in amyloid nucleation.

(A–J) Western blot analysis of SDS-lysed total membranes derived from Mel220 cells stably co-expressing the functional PMEL variant D211A with various PMEL loss-of-function mutants. PMEL-specific antibodies HMB45 (A,B) and I51 (C–J) were used. GM130 serves as loading control in (E–J).