Figure 3. β2-3 contacts are not necessary to describe the SAXS experiment.
(Left) Two separate refinements from the unfolded prior EU, with and without β2-3 states, were performed and the resulting SAXS profiles were compared to experiment. The refined ensembles are identical within experimental error, indicating β2-3 states are not required in the ensemble to give consistency with experiment. The experimental standard deviation due to repeat measurements is represented as the shaded area around the experimental SAXS profile. (Right) A histogram of mean-squared errors between the simulated SAXS profile of each CheY* MSM microstate from the experimental 5-ms SAXS profile indicates that states with the β2-3 feature (blue) are not necessarily in better agreement with experiment compared to states without the feature (red).