Figure 2. Dynasore reduces plasmalogen synthesis.

(A) CHO-K1 cells were incubated with DMSO, Dynasore, or CPZ for 30 min and further metabolically labelled with ¹⁴C-Etn for 5 h in the absence (−) and presence of each inhibitor. The biosynthesis of PE and plasmalogen (2-acyl-GPE) were detected with a FLA-5000 imaging analyser (upper panel). Total cellular level of phospholipids including PE, PS, phosphatidylinositol (PI), plasmalogens, phosphatidylcholine (PC), and SM were detected with iodine vapor (lower panel). Note that biosynthesis of plasmalogens was specifically reduced in the presence of Dynasore. (B) Biosynthesis of PE (open bar), plasmalogens (dark grey bar), PE plus plasmalogens (light grey bar) was represented by taking the amounts of PE plus plasmalogens as 100 in mock-treated (−) CHO-K1. Statistical analysis was performed by one-way ANOVA with Dunnett’s post hoc test as compared with the synthesis of respective lipids in mock-treated cells. *p < 0.05, **p < 0.01, and ***p < 0.001.