Figure 2. In situ hybridization of HpTnI in 3-day-old larvae.

(A) HpTnI is likely expressed around the esophagus, but the signal is not clear from the chromogenic in situ hybridization. The region outlined by the dotted square is magnified in (B). (C,D) Fluorescence in situ hybridization revealed the detailed pattern of HpTnI expression in the esophagus (E), esophageal muscle (EM), anal sphincter (AS) and pyloric sphincter (PS).