Table 1. Chronic Recording Statistics.
| Cohort | Number Inoculated (Day 0) | Mortality Before Day 21 | Number Chronically Recorded | Number Recorded With Epilepsy | Epilepsy Rate (of inoculated) | Epilepsy Rate (of recorded) | Seizure Latency days post inoculation Median (Range) | Mortality After Day 26 | Seizure Associated Mortality | SUDEP |
|---|---|---|---|---|---|---|---|---|---|---|
| SW-PbNK65 | 25 | 4 | 17 | 13 | 0.52 ± 0.10 (p = 1e-11) | 0.76 ± 0.10 (p = 7e-15) | 39 (29–139) | 7 | 7 | 2 |
| SW-PbANKA | 34 | 23 | 10 | 7 | 0.21 ± 0.07 (p = 6e-4) | 0.70 ± 0.14 (p = 4e-8) | 30 (22–105) | 5* | 3 | 1 |
| C57BL/6-PbANKA | 26 | 12 | 12 | 9 | 0.35 ± 0.09 (p = 2e-6) | 0.75 ± 0.12 (p = 2e-10) | 47 (38–95) | 1 | 0 | 0 |
| CBA-PbANKA | 5 | 1 | 4 | 3 | 0.60 ± 0.22 (p = 9e-4) | 0.75 ± 0.22 (p = 3e-4) | 72 (54–116) | 1 | 0 | 0 |
| SW-Control | 9 | 0 | 9 | 0 | 0 (p = 0.7) | 0 (p = 0.7) | 0 | 0 | 0 | |
| C57BL/6-Control | 7 | 0 | 7 | 0 | 0 (p = 0.7) | 0 (p = 0.7) | 0 | 0 | 0 | |
| CBA-Control | 5 | 0 | 5 | 0 | 0 (p = 0.8) | 0 (p = 0.8) | 0 | 0 | 0 |
Statistics from seven cohorts of mouse-strain and parasite/control combinations studied chronically for development of epilepsy from CM. Animals were inoculated at P23 (denoted day 0 here) with infected or uninfected donor blood, then treated with artesenate starting at the peak of cerebral malaria. At time points well past recovery, animals were implanted with electrodes and monitored with continuous video/iEEG recordings. Infected cohorts had between 16–67% mortality rates during the acute infection and recovery time defined up to Day 21, with the highest mortality observed in the SW-PbANKA strain. Listed are the counts of animals recorded that met inclusion criteria to diagnose epilepsy (recorded at least 2 days, alive past day 26, no malaria recrudescence), the number of those that became epileptic (at least 2 seizures longer than 10 s, at least one seizure past day 26), and estimated epileptic rates (±SD estimated from binomial statistics) as fraction of inoculated and as fraction of those surviving to recording. Seizures were not observed, and therefore no epilepsy, among any of the 21 control animals across all three mouse strains. P-values for epilepsy rates were estimated from a null-hypothesis of a rate of 1 epileptic control animal in 22 (Ncontrols + 1) as function of inoculated and recorded numbers. Mortality past day 26 excludes surgical deaths, but includes 2 late malarial recrudescences (denoted with *). Seizure related mortality details counts of spontaneous deaths in seizure animals; SUDEP counts detail animals who died within 2 hours of an electrographic seizure. No early or late mortality was observed in the controls.