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. 2017 Mar 8;7:44134. doi: 10.1038/srep44134

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Polyplexes were prepared in 10 mM HEPES according to the MIXING and DROPPING modes, and diluted in (a) OptiMEM, (b) serum-free DMEM and (c) complete DMEM. D_H of polyplexes was measured by DLS, using the size distribution analysis (CONTIN algorithm) to identify the position of the main peak.