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. 2017 Mar 8;7:44134. doi: 10.1038/srep44134

Figure 3. Effect of centrifugal sedimentation of polyplexes onto cells on transfection efficiency.

Figure 3

(a,c) Ratio between transfection efficiency upon centrifugation (500 × g) of polyplexes onto (a) HeLa cells and (c) HUASMCs and under normal gravitational settling conditions (1 × g). Polyplexes were prepared in 10 mM HEPES (bPEI-H and lPEI-H) and 150 mM NaCl (bPEI-N and lPEI-N) at N/P 30 according to the MIXING and DROPPING procedures, invariably adding pDNA to PEI solution. Results are expressed as mean ± SD, n ≥ 4. **p < 0.01, ***p < 0.001, MIXING vs. DROPPING. (b,d) Ratio between transfection efficiency upon centrifugal sedimentation (500 × g) of polyplexes onto (b) HeLa cells and (d) HUASMCs with respect to normal gravitational conditions (1 × g) expressed in function of polyplex DH. n ≥ 4. Centrifugations consisted in the addition of polyplexes to (a,b) HeLa cells and (c,d) HUASMCs in complete DMEM, followed by prompt centrifugation for 5 min at 500 × g.