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. 2017 Mar 8;7:44134. doi: 10.1038/srep44134

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Ad hoc-prepared 18 mm-diameter glass coverslips featuring three polyethylene cylindrical spacers (1.5 mm high, 1 mm diameter) in (a) upright and (b) upside-down configurations. Schematic representation of the (c) upright and (d) upside-down experimental setups, showing cells facing the top and the bottom of the cell culture well, respectively.