Figure 2. Truncated proteoforms locate up to 8 mannose residues within stretch Gly₃₀-Thr₄₁.

(A) Deconvoluted MS spectrum of the chromatographic peak I attributed to non-lipidated N-terminally truncated LpqH species showing the co-elution of low molecular weight unglycosylated forms (peptidic sequence range from [Gly₅₆-His₁₆₈ to Thr₄₁-His₁₆₈] annotated into brackets) with longer glycosylated forms starting from each color coded N-terminal amino acids figured in the sequence scheme (the colored numbers correspond to the glycosylation degrees of each color coded peptidic sequence). (B) Sequence scheme of the non acylated species of LpqH figuring by dashed lines and colored amino acids respectively the N-terminal residue of the different unglycosylated and glycosylated species detected in the peak I; (Amino acid numbering corresponds to that of the full length (gene) sequence and red diamonds indicate the previously proposed LpqH glycosylation sites⁶).