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. 2017 Mar 8;7:44151. doi: 10.1038/srep44151

Figure 3. Induction of Ag-specific multifunctional T cells in the spleens and lungs in Rv3131-immunised mice.

Figure 3

Each group of mice was immunised and euthanised as described in the Methods section. Four weeks after the final immunisation, the mice in each group were sacrificed, and their lung and spleen cells were treated with Rv3131 (5 μg/mL) at 37 °C for 12 h in the presence of Golgi Stop. (a) The gating strategy used to identify Ag-specific multifunctional T cell populations. (b) Upon stimulation with the Rv3131 vaccine Ag, the percentages of Ag-specific, multifunctional CD4+CD62L− and CD8+CD62L− T cells producing TNF-α, IFN-γ and/or IL-2 in the lung and spleen cells from each immunised group were evaluated using flow cytometry. The mean frequencies of cells producing effector cytokines are shown as pie charts. The results are expressed as the mean ± SD for 5 mice from each group. The significance of differences was determined using an unpaired t-test. A p value < 0.05 was considered statistically significant. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the GLA-SE alone group.