Fig. 5.

Cell cycle effect of HLBT-100. For cell cycle effect determination, MV4-11 leukemic cell line was used. Cells were treated with either DMSO: top row (vehicle) or HLBT-100: middle row (0.024 µM) and bottom row (0.120 µM). Cell were incubated at 37 °C and at 24 h (a), 48 h (b) and 72 h (c), cells were harvested, washed and stained according to the manufacturer’s recommendations with the Abcam propidium iodide flow cytometry kit. Acquired samples were analyzed using FlowJo (Tree Star, Ashland, OR, USA)