Fig. 6.

Effect of HLBT-100 on apoptosis. Apoptotic effect of HLBT-100 was assessed on the MV4-11 leukemic cell line using the Annexin V-FITC apoptosis detection Kit I. After treatment with HLBT-100 (0.024 and 0.120 µM) cells were incubated at 37 °C for 24 h. Cells were harvested and stained with Annexin V-FITC and Propidium Iodide reagents. Stained samples were analyzed using FlowJo (Tree Star, Ashland, OR, USA) for apoptotic effect