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. 2017 Mar 7;17:38. doi: 10.1186/s12935-017-0404-z

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — The Apo-ONE^® Homogeneous Caspase-3/7 Assay (Promega) was used to detect caspase-3/7 activity of HLBT-100 based on the cleavage of a pro-fluorescent DEVD peptide-rhodamine 110 substrate [(Z-DEVD)2-R110] according to manufacturer’s instruction and as reported by Wagner et al. [78]. PC-3 prostate cancer cell line was cultured in F12 K medium supplemented with 10% FBS and 100 µg/ml penicillin, and 100 µg/ml streptomycin. PC-3 cells were treated with HLBT-100 or reference compound staurosporine for 6 h with serum free medium. The activation was considered significant only when the highest compound dose induced caspase-3/7 activity ≥200% compared to DMSO