Fig. 7.

SlVPE3 does not bind to the promoters of genes with abnormal expression in SlVPE3 silenced fruit. For the ChIP assay, genomic DNA and proteins from pericarp of tomato fruit at 41 dpa were cross-linked, and the chromatin complexes were co-immunoprecipitated with anti-SlVPE3 antibodies. The promoter regions of the analyzed genes are indicated. Blue boxes represent the binding motifs and numbers indicate the position of these motifs relative to the translational start site. The green fragments with upper-case letters represent the regions used for ChIP-quantitative PCR. Values represent the percentage of DNA fragments that were co-immunoprecipitated with anti-SlVPE3 antibodies or preimmune serum (rabbit IgG) relative to the input DNA. Error bars represent the standard deviation of three independent experiments