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. 2017 Mar 7;17:26. doi: 10.1186/s12896-017-0344-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Tobacco transient transactivation assay for the interaction between OsbHLH59, OsERF71 and OsXIP promoter. a Characterization of OsXIP promoter and structures of vectors. Full length of the promoter from the translational start site (ATG) was indicated. OP2 and OP3 promoters were constructed into the report vector, and TFs (OsbHLH59, and OsERF71) were cloned into the effect vector, respectively. b Transient expression assay in N. benthamiana. LUC, Firefly luciferase activity; REN, Renilla luciferase activity (used as control). Data show ratios of LUC to REN and represent means ± SD of three independent replicates