Fig. 6. (A) Synthesis of O6-(alkyl)-dGTPs with a Cu(i) carbene derived from EDA. (B) Single nucleotide primer extension with O6-(alkyl)-dGTPs opposite DNA templates G, A, T, and C catalyzed by KTqM747K. Reactions were performed at 55 °C for 10 min and contained 50 μM dNTPs. (C) PAGE analysis of the primer extension experiment. M, blank corresponding to 23 nt primer.
