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(A) Effects of gallamine on the FRET signals of a M2 receptor sensor expressed in HEK293 cells. Gallamine 10 μM reversed the signal induced by 100 μM ACh, reproduced from Maier‐Peuschel et al., 2010. (B) Voltage‐sensitivity of an α2A‐adrenoceptor. The biosensor was activated with 2 μM NA at −90 mV, reflected as a decrease in the FRET signal. Subsequent depolarization to +60 mV deactivates α2A‐adrenoceptor‐cam, indicated by a rise in the FRET ratio. Reproduced from Rinne et al., 2013.
