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. 2015 Dec 16;173(2):319–331. doi: 10.1111/bph.13373

Figure 4.

Figure 4

PDE inhibitors suppressed PMA‐induced NETosis. After human peripheral neutrophils were incubated without or with PMA in the presence of the indicated concentrations of (A) a pan‐PDE inhibitor, IBMX, (B) a PDE3 inhibitor, cilostazol, or (C) a PDE4 inhibitor, rolipram, cells undergoing NETosis were stained with cell‐impermeable DNA stain sytox orange. NETosis was visually measured in more than 300 cells in randomly selected five fields by confocal microscopy. Data shown are expressed as mean ± SEM of cells undergoing NETosis as a percentage of those stimulated with PMA alone in five independent experiments. *P < 0.05 and **P < 0.01 compared with NETosis with PMA alone.