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. 2015 Dec 16;173(2):396–410. doi: 10.1111/bph.13383

Figure 3.

Figure 3

Effects of DHT1 on survival of OCs and bone resorption. OCs generated from CD14+ human mononuclear cells were cultured on bovine bone slices for 72 h (n = 7 per condition). DHT1 (at 1 and 10 μM) and ODN (at 50 and 500 nM) were added at the beginning when OCs had been allowed to attach to the bone surface. After 72 h, bone slices were processed for TRACP positive cells and resorption events quantified. Representative images of TRACP positive cells in (A) ODN‐treated (0, 50 and 500 nM) and (B) DHT1‐treated (0, 1 and 10 μM) cultures (n = 3). (C, D) The morphology of resorption pits and trenches was examined after staining with toluidine blue (n = 4 bone slices per condition). Micrographs of resorption cavities formed by OCs in untreated, DHT1‐ and ODN‐treated cultures at different concentrations. Scale bar (black line) 40 μM.