Figure 2.

The effect of BoNT/A and sumatriptan on Evans blue/plasma protein extravasation in dura mater after TMJ inflammation. BoNT/A was injected into the TMJ (5 U kg⁻¹ i.a.) or trigeminal ganglion (2 U kg⁻¹ i.g.) 3 days before CFA. Sumatriptan (175 mg kg⁻¹) was administered p.o. 24 h after CFA. Four days following BoNT/A or 2 h after sumatriptan rats were injected with Evans blue (i.v., 40 mg kg⁻¹) and perfused with saline. Dura was collected for formamide extraction and spectrophotometric measurement of Evans blue dye which extravasates in complex with plasma proteins. Scatter plot represents data from individual animals, and horizontal lines and bars indicate mean ± SEM. n (animals per group) = 5–9. *P < 0.05, ***P < 0.001, significantly different from saline control; ⁺⁺ P < 0.01; ⁺⁺⁺ P < 0.001, significantly different from saline + CFA; one‐way ANOVA followed by Newman–Keuls post hoc test.