Figure 8.
BoNT/A antinociceptive activity and occurrence of cleaved SNAP‐25 in dura mater is dependent on axonal transport. (A) Preventive effect of i.a. BoNT/A (5 U kg−1) on mechanical allodynia evoked by CFA injection into the TMJ is prevented by colchicine (5 mM) injection into the trigeminal ganglion. Scatter plot represents individual animal values, and horizontal lines and bars indicate mean ± SEM. n (animals per group) = 5–6. ***P < 0.001, significantly different from saline i.a. + CFA; +++ P < 0.001, significantly different from BoNT/A i.a. + CFA; one‐way ANOVA followed by Newman–Keuls post hoc test. (B) Colchicine prevented the occurrence of cleaved SNAP‐25 immunofluorescence in dura mater. The image is representative of the data obtained from four animals per group. Scale bar = 100 μm.