Figure 5.

α₁‐Adrenoceptor signalling mediated Cebpd expression. (A) Cells were treated with 10 μM phenylephrine (PHE) for 1, 2, 4 and 8 h, harvested and then processed for real‐time qRT‐PCR. Each value represents the mean ± SEM of six separate experiments. ^* P < 0.05, significantly different from each control value obtained in MC3T3‐E1 cells cultured in the absence of PHE. (B) α₁‐Adrenoceptor signalling mediated Cebpd mRNA expression after the PHE stimulation in MC3T3‐E1 cells. Cells were incubated for 2 h in the presence of PHE with prazosin at a concentration of 10 μM, followed by the determination of Cebpd levels by real‐time qRT‐PCR. Each value represents the mean ± SEM of six separate experiments. ^* P < 0.05, significantly different from each control value. NS; not significant. (C) α₁‐Adrenoceptor signalling regulated Cebpd in bone. Effects of the systemic administration of prazosin on Cebpd mRNA expression in cancellous bone. Total RNA was isolated from the distal region of the femur in 10‐week‐old male mice administered saline or prazosin, followed by the determination of Cebpd mRNA levels by real‐time qRT‐PCR using specific primers; n = 7 or 8 mice per group. Values are expressed as means ± SEM. ^* P < 0.05, significantly different from each control value. (D) Total RNA was isolated from the distal region of the femur from WT and α_1B ^−/− mice, followed by the determination of Cebpd mRNA levels by real‐time qRT‐PCR using specific primers. n = 7 or 8 mice per group. Values are expressed as means ± SEM. ^* P < 0.05, significantly different from WT mice.