Figure 1.

Effect of Compound 9a on the cytokine and NO release in LPS‐stimulated RAW264.7 cells. For the cellular toxicity assay, RAW264.7 cells were treated with vehicle, various concentrations of Compound 9a or suberoylanilide hydroxamic acid (SAHA) for 24 h (A). To measure cytokine release, RAW264.7 cells were treated with vehicle, various concentrations of Compound 9a or SAHA for 1 h before LPS (1 μg∙mL⁻¹) stimulation. Twenty‐four hours after LPS stimulation, release of TNF‐α (B), IL‐6 (C) and NO (D) was measured. The results are presented as mean ± SEM of independent experiments. ^* P < 0.05, significantly different from control. ^† P < 0.05, significantly different from vehicle.