Table 1.
Oligonucleotides used as PCR primers
| Gene | Forward Primer (5′ to 3′) | Reverse Primer (5′ to 3′) | Product size (bp) | References |
|---|---|---|---|---|
| Rat MyoD | GGAGACATCCTCAAGCGATGC | AGCACCTGGTAAATCGGATTG | 105 | (Gomes et al., 2006) |
| Rat myogenin | ACTACCCACCGTCCATTCAC | TCGGGGCACTCACTGTCTCT | 233 | (Kamikawa et al., 2013) |
| Rat 18S rRNA | CACGGGTGACGGGGAATCAG | CGGGTCGGGAGTGGGTAATTTG | 105 | (Kim et al., 2011) |
| Rat ETA receptor | ATGGGTGTCCTTTGCTTTCTGGCGT | TTAGTTCATGCTGTCCTTGTGGCTG | 1281 | – |
| Rat Gq protein | ATGACTCTGGAGTCCATCATGGCGT | TTAGACCAGATTGTACTCCTTCAGG | 1080 | – |
| Rat G11 protein | ATGACTCTGGAGTCCATGATAGCGT | TCAGACCAGGTTGTACTCCTTCAGG | 1080 | – |
| Rat GRK2‐ct | GCCAAAAACAAACAGCTGGGC | ACTGCCACGCTGAATCAGTGG | 432 | – |
| Rat InsR | CAGGATTGGCGCTGTGTAAAC | CACAGCTGCCTCAGGTTCTG | 491 | (Obici et al., 2002) |
| Rat Akt2 | ATGAATGAGGTATCTGTCATCAAAG | TCACTCTCGGATGCTGGCTGAGTAG | 1446 | – |
| Rat GLUT4 | ATGCCGTCGGGTTTCCAGCAGATCGGC | TCAGTCATTCTCATCTGGCCCTAAGTATTC | 1530 | – |