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. 2016 Feb 17;173(6):1005–1017. doi: 10.1111/bph.13404

Figure 2.

Figure 2

Concentration–response curves to BK in the presence of Indo + TRAM34 + apamin (A, inhibitors of COX, KCa3.1 and KCa2.x channels) in endothelium‐intact mesenteric arteries of rats treated with placebo, 48 h or 72 h of continuous i.v. infusion of serelaxin. n = 7–10 per group. (B) Basal NOS activity in endothelium‐intact mesenteric arteries of rats treated with placebo, 48 or 72 h of continuous i.v. infusion of serelaxin. In each group of experiments, mesenteric arteries were pre‐contracted with phenylephrine to a similar level (~20% of KPSS contraction) before the addition of L‐NAME (200 μmol·L−1), n = 8 per group. Western blot analysis of total eNOS (C), phospho‐eNOSser1177 (D), iNOS (E) and RXFP1 (F) protein expression in mesenteric arteries of rats treated with placebo, 48 h or 72 h of continuous i.v. infusion of serelaxin. Representative blots are shown above their respective panels. n are shown in parentheses. * P < 0.05; significantly different from placebo: # P < 0.05; significantly different from 48 h serelaxin treatment: one‐way anova, Tukey's post hoc test.