Table 5.
Culture conditions, 1969–1978.
| Year | LOR with documented egg recoverya (n) (% of stimulated cycles started) | Eggs retrieved (n) | Medium 1: flushing, egg wash, sperm wash, fertilization | Medium 2: cleavage/embryo transfer | Cycles with insemination (n) (% of LOR with egg recovery) [eggs inseminated (n), % of eggs recovered] | Cycles with embryos reported (n) (% of LOR with egg inseminated) [bTotal embryos (n), % of eggs inseminated] |
|---|---|---|---|---|---|---|
| 1969 | 48 (83) | 136 | No records until Sept; M199, Barry’s B + BSA or HSA; FF: 10%, 20%, 50% | Minitube culture, 5% CO2 in air; M199, Hank’s+ FCS, BSA, HSA, 2.5%; | 7 (15) [27, 20] | 1 (14) [1, 4] |
| 1970 | 55 (89) | 274 | Microdrop culture, 5% CO2 60% FF/Barry’s B, ‘special B’ (high pH), + Na/K Whittingham’s/Weymouth’s 1:1 , 15% FCS TyrB + 0.36% BSA, 1%BSA; + Pyr, lactate, bicarb; Tyr B minus extra bicarb + Heparin 10 IU/ml; Whittingham’s + 20% FCS | Whittinghams + incr. Na/K; Ham’s F10 + 0.36 g% BSA, or 20%, 40% FCS M199 + pyr, lact, bicarb, BSA M199 + 10 % FCS + 5%PO4 + 100iuml bicarb; Ham’s F12 + 20%FCS or 0.72% BSA + 1x L-Glutamine Reduced O2 in May; Aug: 20% human serum | 51 (93) [272, 99] | 25 (49) [36, 13; + 3 × 2PN] |
| 1971 | 36 (88) | 175 | Tyr + Na/K for sperm and fertilization, + 0.36% BSA; Tyr for eggs:minus extra bicarb, + PO4 + Hep for flushing Streptomycin added for sperm | Ham’s F10, F12, + 20%FCS (inactivated), + 5%, 10%, 20% HS 5% CO2 in air, 5 % O2/5 % CO2/90 % N2 from September; Oldham vs Cambridge water compared | 36 (100) [175, 100] | 13 (36) [18, 10 + 1 × 2?PN] |
| 1972 | 39 (95) | 187 | No phenol red in Tyrode’s; TyrB ± bicarb, BSA/FCS/HS + Pyr, Penicillin Stock solutions made. Autoclaved BDH water; 5 % O2/5 % CO2/90 % N2 | Ham’s F10 + L-Glutamine + 20 % FCS + 5 % HS; Sept: Ham’s F10 + 20% maternal serum collected at beginning of laparoscopy | 35 (90) [179, 96] | 10 (29) [15, 8; + 6 × 2PN & 1 × Syngamy] |
| 1973 | 25 (57) | 86 | New batches Pyr, BSA, BDH water in glass or plastic bottles, SVT for different paraffins & sera, FCS tested with HeLa & GC cultures | As previously – serum inactivated/not inact., Maternal serum, Paternal serum; F10 minus bicarb for ET | 20 (80) [81, 95] | 8 (40) [8, 10; 2 × 2PN, 3 × ?2PN] |
| 1974 | 28 (76) | 85 | As previously, SVT for different sources of HSA; extra Ca & Mg added in June | As previously, new F10 for ET: no BSA or bicarb | 28 (100) [81, 95] | 10 (36) [15,18; + 1 × 2PN] |
| 1975 | 33 (92) | 117 | As before, extra Ca, Mg, P; McCoy’s modified w/bicarb September: EBSS + glucose, BSA, Pyr, Pen. Several SVTs for different batches of media, sera, maternal serum Nov: Tyrode compared with Hoppit’s by SVT Dec: non-autoclaved water, EBSS + Pyr, 0.21% bicarb, BSA, HS, | F10 reinforced: + Ca, Mg, Glutamine, bicarb. No bicarb for ET. Laparoscopy plasma, inactivated serum. All tested with SVT | 33 (100) [117, 100] | 15 (46) [17, 15; + 1 × 2PN] |
| 1976 | 44 (80) | 147 | Earle’s for flushing, Fert, Sperm wash + Pyr, Pen, ± bicarb | Ham’s F10 + bicarb, glutamine, Pen. FF assays, GC cultures, ‘serum new method’, inactivated + not inactivated; F10 for cleavage and ET. All checked for osmolarity | 44 (100) [149, 100] | 29 (66) [42, 29] |
| 1977 | 28 (65) | 97 | As 1976, Earles Fert & Hep Earle’s Alb, + 0.36% BSA. Osmometer checked with new standards: all media adjusted to 279–273 | As 1976, F10 ‘serum new method; FF assays, GC cultures, serum not inactivated | 27 (96) [95, 98] | 22 (82) [20 SC; 2 NC][35, 37] |
| 1978 | 52 (67) | 57 | As 1977 | As 1977; FF assays, GC cultures | 50 (96) [60, 98] | 34 (68) [34, 57] |
| Total | 388 (78) | 1361 | 331 (85) [1237/1361, 91] | 165 (51) [221/1237; 18] |
ET = embryo transfer; FCS = fetal calf serum; FF = follicular fluid; LOR = laparoscopic oocyte recovery; NC = natural cycle; SC = stimulated cycle
a
Cases for laparoscopic sterilization not included