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. 2016 Nov 14;7(51):83837–83842. doi: 10.18632/oncotarget.13335

Figure 1. Variable ectopic mineralization patterns in dermal sheath of vibrissae, outer ear, aorta, and trachea of asj-2J mice.

Figure 1

Left panel: Ectopic mineralization is noted in the dermal sheath of vibrissae, elastic cartilage of outer ear, aorta, and hyaline cartilage of trachea (white arrow). Middle panel: Green (calcein, green arrow) and red (alizarin complexone, red arrow) fluorescent labels are noted in vibrissae, when administered at 4 and 10 weeks, respectively. Green (calcein, green arrow), red (alizarin complexone, red arrow), and yellow (demeclocycline, yellow arrow) fluorescent labels are noted in outer ear, when administered at 4, 10, and 11 weeks, respectively. No mineralization labels are found in aorta and trachea. Right panel: Positive TRAP activity is found in trachea only (yellow arrowhead). TB, Toludine Blue O which stains the anatomic structure of tissues. CB, Calcein Blue which stains accumulated minerals. DAPI stains nuclei blue. AP, alkaline phosphatase activity of osteoblasts (red). TRAP, tartrate-resistant acid phosphatase activity of osteoclasts (yellow).