Table 1. Tumor cell lysis by irradiated haNK cells, and tumor cell expression of MICA, PD-L1, and MHC class I.
| Tumor cell line | Tumor type | % MICA (MFI) | % PD-L1 (MFI) | % MHC I (MFI) | % haNK lysis (SD) |
|---|---|---|---|---|---|
| CaSki | Cervical | 99.3 (10,229) | 99.9 (20,015) | ND | 46.5 (4.9) |
| HN30 | Head & neck | 2.3 (12,995) | 99.8 (21,328) | 98.7 (10,963) | 45.3 (3.9) |
| HTB1 | Bladder | 73.1 (4,755) | 98.4 (17,225) | 99.5 (10,154) | 37.6 (2.5) |
| HN4 | Head & neck | 25.2 (8,842) | 99.7 (10,005) | 99.5 (9,232) | 34.0 (5.3) |
| HN12 | Head & neck | 36.8 (27,815) | 100 (5,177) | 96.5 (9,603) | 31.0 (2.0) |
| Colo205 | Colorectal | 7.25 (2,001) | 95.7 (1,765) | 98.6 (745) | 23.4 (2.1) |
| SW403 | Colorectal | 2.3 (10,176) | 51.3 (5,176) | ND | 8.6 (2.7) |
| ASPC-1 | Pancreatic | 0.3 (8,592) | 24.0 (3,101) | 98.8 (3,060) | 0 (1.5) |
Tumor cell lines were phenotyped by flow cytometry and used as targets in 18 h 111In-release haNK lysis assays using irradiated (10 Gy) haNK as effectors. The effector to target ratio was 10:1. MICA, PD-L1 and MHC class I expression are shown as % positive cells (MFI). ND = not done.