Table 1.

Amino acid (AA) relative frequency (%) and conservation index (CI)

	IM					TM					M
AA	N	%	CI	tM	D	N	%	CI	tM	D	N	%	CI	tM	D
A	37	5.1	50.4	37	0	192	8.3	57.4	177	7	26	3.5	44.6	27	1
C	3	0.4	67.4	1	0	14	0.6	59.2	8	0	5	0.7	64.5	4	0
D	28	3.8	73.2	25	0	21	0.9	69.8	9	2	17	2.3	72.4	16	0
E	18	2.5	57.5	7	0	42	1.8	78.4	23	2	28	3.8	71.0	15	2
F	30	4.1	60.7	22	0	154	6.7	69.5	104	1	32	4.3	58.3	18	1
G	46	6.3	73.9	25	0	128	5.5	80.8	60	4	38	5.1	77.0	15	0
H	18	2.5	64.6	12	0	51	2.2	74.0	22	1	28	3.8	55.9	26	0
I	45	6.2	44.4	44	0	234	10.1	46.0	318	0	40	5.4	35.5	55	0
K	11	1.5	58.2	1	0	39	1.7	66.2	9	0	45	6.0	45.3	17	0
L	98	13.4	50.1	44	1	457	19.7	60.4	205	12	89	11.9	49.0	46	1
M	31	4.3	52.5	20	2	140	6.1	38.9	134	3	37	5.0	34.1	36	0
N	49	6.7	48.4	49	0	65	2.8	47.7	59	0	50	6.7	44.0	70	0
P	61	8.4	70.9	31	0	90	3.9	65.1	41	2	68	9.1	65.2	41	0
Q	24	3.3	55.9	8	0	35	1.5	63.2	11	1	31	4.2	54.1	20	0
R	10	1.4	86.6	2	0	33	1.4	80.5	14	1	20	2.7	82.6	13	3
S	58	8.0	39.6	55	0	159	6.9	53.1	117	2	57	7.6	42.2	61	2
T	82	11.2	36.3	73	0	207	8.9	40.6	201	0	62	8.3	33.4	79	0
V	33	4.5	49.7	32	0	114	4.9	50.7	147	3	20	2.7	42.0	18	0
W	20	2.7	89.0	1	0	64	2.8	79.9	19	1	20	2.7	71.5	7	0
Y	27	3.7	67.1	17	1	75	3.2	64.9	36	0	33	4.4	43.2	31	1
	729			506	4	2314			1714	42	746			615	11

IM, TM, M, N, tM and D code for intermembrane, transmembrane, and matrix domains, total positions with this amino acid, number of total and damaging mutations, respectively