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. 2017 Mar 7;14:47. doi: 10.1186/s12985-017-0716-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — East African Cassava mosaic virus (EACMV-K201) infectious clone-based virus-induced gene silencing (VIGS) vector. a Coat protein (CP) nucleotide sequence of EACMV-K201 DNA-A showing position and mutated nucleotides that introduced Avr II and Nhe I near 5′-end and Sbf I near 3′-end. Nucleotide positions were counted from the start codon “ATG” of the CP. b Infectivity of the GFP-VIGS and MePSY2-VIGS targeting GFP and cassava phytoene synthase coding sequences, respectively, in wild-type cassava cultivar TME 7S. GFP-VIGS has no known target in the challenged cassava plant, and these plants display typical mosaic symptoms characteristic of CMD on leaves. MePSY2-VIGS-challenged plants show chlorosis on leaves. Pictures were collected at 2 and 12 weeks post inoculation (WPI)