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. 2017 Mar 7;14:47. doi: 10.1186/s12985-017-0716-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Virus titer in wild-type and FEC-derived TME 7 (Oko-iyawo) plants challenged with GFP-VIGS and MeSPY1-VIGS at 9 DPI. a Virus titer determination by Southern blot and b qPCR performed on total DNA extracted from leaves of wild-type (resistant to CMD) and FEC-derived (susceptible to CMD) plants. c RT-qPCR expression analysis of MeSPY. Higher virus titer is detected in both GFP-VIGS and MeSPY1-VIGS-challenged FEC-TME7 (susceptible plants) compared to the wild-type TME 7. Primers used for labelling probes for Southern blotting and for qPCR and RT-qPCR assays are shown in Table 1. Bars show SE (n = 4). P, positive control, N genomic DNA from unchallenged plants. Samples from three infected plants were pooled to make one sample, and a total of 4 samples (from 12 plants) were used per treatment combination