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Additional file 1: Time-lapse video showing response of wild-type TME 204 (resistant to cassava mosaic disease, CMD) and FEC-TME 204 plants (susceptible to CMD) to inoculation with MeSPY1-VIGS. Both plant types were challenged with the modified EACMV-K201 (MeSPY1-VIGS) along with infectious DNA B clone. Images of challenged plants were captured every hour beginning from the 5^th day after inoculation for a total of 22 days using 5MP camera boards controlled by Raspberry Pi microcomputers. Images were then converted to a movie file using Apple iMovie. Only pictures collected every 2 hours and during daytime were presented. Note the susceptible FEC-TME 204 plants die while the wild-type TME 204 survive MeSPY1-VIGS challenge. (M4V 14035 kb)