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. 2016 Oct 7;150(1):115–126. doi: 10.1111/imm.12672

Figure 2.

Figure 2

Treatment with glucocorticoid (GC) is able to improve intestinal architecture and to modulate inflammatory infiltrate in intestine. C57BL/6 mice were exposed to dextran sulphate sodium (DSS) 3% and treated with dexamethasone as described in the Material and methods. The colon was collected on the 6th day for histopathological analysis and cell immunophenotyping. (a) Left panel – control mice, not exposed to DSS; centre panel – DSS, mice exposed to DSS; right panel – DSS+GC, mice exposed to DSS and treated with glucocorticoids. (b) N‐acetylglucosaminidase (NAG), (c) eosinophil peroxidase (EPO) and (d) myeloperoxidase (MPO) activities. For phenotypic characterization, leucocytes were isolated from (e) lamina propria (LP) and (f) intraepithelial lymphocyte (IEL) compartments. After acquisition, samples were analysed by flowjo software. DSS: C57BL/6 mice exposed to DSS; DSS+GC: C57BL/6 mice exposed to DSS and treated with glucocorticoids. The dashed lines correspond to the group of C57BL/6 control mice not exposed to DSS. These results are representative of three independent experiments with five mice/group. Data were analysed using one‐tailed unpaired t‐tests in (c), (e) and Mann–Whitney one‐tailed tests in (b), (d). *P < 0·05.