Figure EV2. Promotion of gallbladder organoid growth by R‐spondin 1, noggin, and a TGFβ receptor kinase inhibitor.

1. Representative images of EdU‐stained gallbladder organoids cultured in media with or without R‐spondin 1 and noggin, as indicated (R, R‐spondin 1; N, noggin; +, presence; −, absence). The nuclei were counterstained with DAPI. Scale bar: 200 μm.
2. Phase‐contrast images of gallbladder‐derived organoids cultured with a TGFβ receptor kinase inhibitor (TRKI) for 2 months in the presence (+) or absence (−) of R‐spondin 1 (R) and/or noggin (N), as indicated. Scale bar: 200 μm.