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. 2016 Feb 22;17(5):753–768. doi: 10.15252/embr.201541866

Figure 8. Tf2 retromobility assay.

Figure 8

  1. Pictures of YES plates with G418 showing growth of G418‐resistant subclones in fft3Δ (PP3) and fft2Δfft3Δ (PP4) mutant strains with new integrations of Tf2‐12‐neoAI. Pictures were taken after 3 days of growth at 32°C.
  2. A table showing the Tf2 transposition frequencies and rates calculated with fluctuation tests using FALCOR.
  3. Southern blot probed for neoA in fft3Δ (PP3) and fft2Δfft3Δ mutant strains (PP4) and derived G418‐resistant subclones. The bands marked by asterisks represent new unique Tf2 insertions.