Table 1.
Antibodies used for immunohistochemistry
| Antibody | Product code | Specificity | Genea | Host/clone | Dilution | Source |
|---|---|---|---|---|---|---|
| Dystrophin | GTX15277 | Human dystrophin (C‐terminus) | DMD | pAb‐rabbit | 1 : 7500 | 1 |
| Dystrophin | NCL‐DYS1 | Human dystrophin (rod domain) | DMD | mAb‐mouse/Dy4/6D3 | 1 : 5 | 2 |
| Dystrophin | NCL‐DYS2 | Human dystrophin (C‐terminus) | DMD | mAb‐mouse/Dy8/6C5 | 1 : 10 | 2 |
| Dystrophin | NCL‐DYS3 | Human dystrophin (N‐ terminus) | DMD | mAb‐mouse/DY10/12B2 | 1 : 10 | 2 |
| Desmin | M0760 | Human and animal desmin | DES | mAb‐mouse/D33 | 1 : 100 | 3 |
| Desmin | 18‐0016 | Human desmin | DES | mAb‐mouse/ZC18 | 1 : 1000 | 4 |
| Desmin | ab15200 | Human and animal desmin | DES | pAb‐rabbit | 1 : 2000 | 5 |
| Laminin | PC 128 | Human laminin | LAM | pAb‐sheep | 1 : 15000 | 6 |
| βeta‐Dystroglycan | NCL‐b‐DG | Human beta‐dystroglycan | DAG1 | mAb‐mouse/43DAG1/8D5 | 1 : 500 | 2 |
| Caveolin‐3 | 610421 | Animal caveolin‐3 | CAV3 | mAb‐mouse/26/caveolin‐3 | 1 : 500 | 7 |
| nNOS | AB5380 | Human and animal nNOS, NOS‐I, bNOS | NOS1 | pAb‐rabbit | 1 : 5000 | 8 |
| Vinculin | V9131 | Human vinculin | VCL | mAb‐mouse/hVIN‐1 | 1 : 5000 | 9 |
| H‐Nestin | MAB1259 | Human nestin | NES | mAb‐mouse/196908 | 1 : 250 | 10 |
| Utrophin | sc‐33700 | Human utrophin | UTRN | mAb‐mouse/8A4 | 1 : 200 | 11 |
| α‐Actinin | ab9465 | Human and animal α‐actinin (sarcomeric) | ACTN3 | mAb‐mouse/EA‐53 | 1 : 5000 | 9 |
| Spectrin | NCL‐SPEC2 | Human and animal spectrin | SPBT | mAb‐mouse/RBC1/5B1 | 1 : 1000 | 12 |
| Vimentin | sc‐6260 | Human vimentin | VIM | mAb‐mouse/V9 | 1 : 250 | 11 |
| MyHC | A4.840 | MyHCI | MYH7 | mAb‐mouse | 1 : 20 | 13 |
| MyHC | N2.261 | MyHCI | MYH7 | mAb‐mouse | 1 : 400 | 13 |
| MyHCIIa | MYH2 | |||||
| MyHCeom | MYH13 | |||||
| MyHCα‐cardiac | MYH6 | |||||
| MyHC | MyHC‐slow tonic | MYH14 | pAb‐rabbit | 1 : 500 | 14 |
Official gene nomenclature according to OMIM (http://www.ncbi.nlm.nih.gov/omim/).
1. GeneTex, Taiwan; 2. Novocastra Laboratories, UK; 3. Dako, Sweden; 4. Invitrogen, CA, USA; 5. Abcam, UK; 6. Binding Site, USA; 7. BD Biosciences, USA; 8 Chemicon, CA, USA; 9. Sigma‐Aldrich, UK; 10. R&D Systems, UK; 11. Santa Cruz Biotechnology, UK; 12. Leica Biosystems, UK; 13. Developmental Studies Hybridoma Bank, developed under the auspices of the NICHD and maintained by the University of Biological Sciences, Iowa City, Iowa, USA; 14. gift from Stefano Schiaffino, CNR Inst of Neuroscience, Padova, Italy.
DMD, Duchenne muscular dystrophy; mAb, monoclonal antibody; nNOS, neuronal nitric oxide synthase; pAB, polyclonal antibody.