Figure 4. Double staining for activin A and the microglia marker IBA1 in the NAc.
(a) Representative images of immunofluorescence staining in the NAc after cocaine (COC) or saline (SAL) self-administration during the binge test (AC: anterior commissure). (b) The number of IBA1+ cells in the NAc. (c) Percentage of IBA1+ cells exhibiting an activated morphology. (d) Percentage of IBA1+ cells that double-stained for activin A. Data are expressed as mean ± SEM, *P < 0.05, #P < 0.1 vs saline, n = 4/group.