Table 5.

Quantitative RT‐PCR results from primary human FACS isolated cells obtained from skeletal muscle

	MH	Pre‐FACS	CD56+	CD90+	Lin+
Pax7	1	<4.4	80	<0.9	<1.3
Thy‐1/CD90	1	<1.9	0.3	55	<0.6
COL1A1	1	11	2	53	0.2

Pax7, Thy‐1 (CD90) and COL1A1 mRNA expression in whole muscle homogenate (MH), pre‐FACS isolation sample (Pre‐FACS), isolated CD56⁺/CD90⁻/Lin‐ [CD45/CD31⁻] cells (CD56⁺), CD90⁺/CD56⁻/Lin⁻ cells (CD90) and CD45⁺/CD31⁺/CD56⁻/CD90⁻ cells (Lin⁺). Data are presented as cDNA molecules normalized to ribosomal protein large P0 expression and further normalized to expression level in MH (n = 1 included in analysis). When below detection level (one molecule) this was indicated by ‘<’, followed by the calculated value for one cDNA molecule.