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A–D, phosphorylation of ACC at Ser‐221 (A), ULK1 at Ser‐555 (B), protein content of LC3‐II (C) and LC3‐II/LC3‐I ratio (D) in human myotubes incubated either with or without AICAR for 30 min. E, representative Western blots; n = 5. Data are presented as means ± SEM. *Significantly different (P<0.05) from Ctrl. AICAR, 5‐aminoimidazole‐4‐carboxamide riboside; AU, arbitrary units; Ctrl, control.
