TABLE 1:
Design of TALEN.
| Gene | Exon | Talen-L (5′ to 3′) | Talen-R (5′ to 3′) | Primers | Digestion |
|---|---|---|---|---|---|
| Mff | 4 | CTATGAGTGAAAATGC | CATTTCTGACCAGCTG | Forward: CAACCGTAGAAGCCAGGAAG | PvuII cuts into 488, 280 base pairs |
| Reverse: AGCTGGAAAGGACCAGTTCA | |||||
| Opa1 | 3 | GGGAAATTGATGAGTA | ATTTTAATGTTCATGA | Forward: AGGGCAAAATTATGAAACCTG | ClaI cuts into 220, 336 base pairs |
| Reverse: AAAATGCTCCAAACAGTTAGTGG | |||||
| Drp1 | 2 | CAGTGCTAGAAAGCCTGG | GACAATTCCAGTACCTC | Forward: TGAAACCTGGTTGGTGGAGT | EcoO109I cuts into 244, 596 base pairs |
| Reverse: AAAGATGCATACTACTTCTCACAGG |